P-43: Short Term Effect of Oxytocin on Testis under Ischemia Reperfusion Injury

Background: The aim of this study was to investigate the short term protective effect of oxytocin on testicular ischemia reperfusion injury immediately after detorsion in a rat model. Materials and Methods: Twenty adult rats were randomly divided into four groups: Control, Ischemia- Reperfusion (IR), Oxytocin and IR+ Oxytocin. Testicular ischemia was achieved by torsion of the left testis 720° clockwise for 2 hours. Two hours after ischemia, torsion was removed and reperfusion was performed. Immediately after reperfusion 0.03 μg/kg oxytocin were administered intraperitoneally to the IR+ Oxytocin group. Three hours after surgery left testis were removed and evaluations were made by Jhonson&#039;s score and ELISA for study of maturation of seminiferous tubules and assay of serum testosterone, FSH and LH levels respectively. Results: Torsion of testis in IR group for two hours induced degeneration of germ cells, edema and blood vessels congestion in testis. A significant reduced Jhonson&#039;s score were detected in IR group in compare with controls (p< 0.05). However there was not significant statistical difference in serum levels of testosterone, FSH and LH. Administration of oxytocin in IR+ Oxytocin group immediately after reperfusion, improved testicular histology in compare with IR group without any effect on levels of abovementioned hormones. Conclusion: Exogenous oxytocin in short term after testicular reperfusion has a protective effect on testis.